primary antibody against p-jak2  (ABclonal Biotechnology)

Journal: Open Medicine

Article Title: Mechanisms of hesperetin in treating metabolic dysfunction-associated steatosis liver disease via network pharmacology and in vitro experiments

doi: 10.1515/med-2025-1215

Figure Lengend Snippet: Pattern diagram of molecular docking: (a) Hesperetin-IL-6, (b) hesperetin-CYCS, (c) hesperetin-TGFB1, (d) hesperetin-IL1B, (e) hesperetin-SOCS3, and (f) hesperetin-CEPBA.

Article Snippet: The primary antibodies against interleukin-6 (IL-6) (DF6087), p-JAK2 (AF3024), JAK2 (AF6022), p-STAT3 (AF3293), and STAT3 (AF6294) were purchased from Affinity Biosciences (OH, USA).

Techniques:

Journal: Open Medicine

Article Title: Mechanisms of hesperetin in treating metabolic dysfunction-associated steatosis liver disease via network pharmacology and in vitro experiments

doi: 10.1515/med-2025-1215

Figure Lengend Snippet: Effects of hesperetin on IL-6-STAT3-SOCS3 pathway in FFA-induced HepG2 cells. (a) IL-6 content in cell culture medium. (b) Relative protein levels of IL-6 in HepG2 cells. (c) Relative protein levels of p-STAT3/STAT3 and SOCS3 in HepG2 cells. (d) Glucose absorption in different groups. (e) Levels of IL-6 in HepG2 cells. (e) Relative protein levels of p-STAT3/STAT3 and SOCS3 in IL-6-induced HepG2 cells. The data represented as mean ± SD, n = 3. * P < 0.05, ** P < 0.01, compared to Ctrl; # P < 0.05, ## P < 0.01, compared to FFA.

Article Snippet: The primary antibodies against interleukin-6 (IL-6) (DF6087), p-JAK2 (AF3024), JAK2 (AF6022), p-STAT3 (AF3293), and STAT3 (AF6294) were purchased from Affinity Biosciences (OH, USA).

Techniques: Cell Culture

Journal: Journal of Inflammation Research

Article Title: N-Ethyl-N-Nitrosourea Induced Leukaemia in a Mouse Model: Protective Effect of Icaritin via Inhibition of IL-6/JAK2/STAT3 Pathway Causes Apoptosis

doi: 10.2147/JIR.S441755

Figure Lengend Snippet: Effect of ICT on IL-6/JAK2/STAT3 in ENU-induced leukemic mice ( A ) IL-6; ( B ) JAK-2; ( C ) STAT 3 in spleen and liver. Data are mean ± SD (n = 6). *P < 0.01 vs normal control, # P < 0.01 vs Leukemic group.

Article Snippet: To perform immunofluorescence experiments, the 5μm thick tissue sections were incubated with primary antibodies against JAK2, p-JAK2, STAT3, p-STAT3 (Cell Signaling Technology (CST), Danvers, Massachusetts, USA) at a dilution of 1:1000 for one hour at room temperature.

Techniques: Control

Journal: Journal of Inflammation Research

Article Title: N-Ethyl-N-Nitrosourea Induced Leukaemia in a Mouse Model: Protective Effect of Icaritin via Inhibition of IL-6/JAK2/STAT3 Pathway Causes Apoptosis

doi: 10.2147/JIR.S441755

Figure Lengend Snippet: Immunofluorescence analysis of proteins in the JAK-STAT pathway in spleen sections from ENU-leukemia mice of ( A ) JAK2 (green) and p-JAK2 (red) staining, showing higher levels of JAK2 phosphorylation in ENU-leukemia mice compared to control mice., ( B ) STAT3 (green) and p-STAT3 (red) staining, indicating enhanced STAT3 signaling in ENU-leukemia mice compared to control mice. Scale bar= 50 µm, Magnification= 400 ×.

Article Snippet: To perform immunofluorescence experiments, the 5μm thick tissue sections were incubated with primary antibodies against JAK2, p-JAK2, STAT3, p-STAT3 (Cell Signaling Technology (CST), Danvers, Massachusetts, USA) at a dilution of 1:1000 for one hour at room temperature.

Techniques: Immunofluorescence, Staining, Phospho-proteomics, Control

Journal: International Journal of Ophthalmology

Article Title: Morroniside ameliorates lipopolysaccharide-induced inflammatory damage in iris pigment epithelial cells through inhibition of TLR4/JAK2/STAT3 pathway

doi: 10.18240/ijo.2023.12.03

Figure Lengend Snippet: The expression of TLR4, JAK2, p-JAK2, STAT3, and p-STAT3 in cells (n=3). Data were represented as mean±SD of three independent samples. The data in Western blot assays were showed after being normalized to β-actin. aP<0.01 compared with the control group, bP<0.05, cP<0.01 compared with the LPS group.

Article Snippet: The membranes were then incubated with against TLR4, JAK2, p-JAK2, STAT3 and p-STAT3 primary antibodies (dilution ratio 1:1000, Abclonal, Wuhan, China) at 4°C overnight.

Techniques: Expressing, Western Blot, Control